Proteintech, 12979-1-AP, GABRA4 Polyclonal antibody

Size: 20ul / 150ul The GABRA4 (12979-1-AP) by Proteintech is a Polyclonal antibody targeting GABRA4 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 12979-1-AP targets GABRA4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue, mouse brain tissue, human kidney tissue, mouse kidney tissue Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABRA4, encoding the extrasynaptically located α4‐subunit of the GABAA receptor, is involved in the etiology of autism. (PMID: 32028044, 32033586) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3600 Product name: Recombinant human GABRA4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 338-554 aa of BC035055 Sequence: AAVNYFTNIQMEKAKRKTSKPPQEVPAAPVQREKHPEAPLQNTNANLNMRKRTNALVHSESDVGNRTEVGNHSSKSSTVVQESSKGTPRSYLASSPNPFSRANAAETISAARALPSASPTSIRTGYMPRKASVGSASTRHVFGSRLQRIKTTVNTIGATGKLSATPPPSAPPPSGSGTSKIDKYARILFPVTFGAFNMVYWVVYLSKDTMEKSESLM Predict reactive species Full Name: gamma-aminobutyric acid (GABA) A receptor, alpha 4 Calculated Molecular Weight: 554 aa, 62 kDa Observed Molecular Weight: 62-70 kDa GenBank Accession Number: BC035055 Gene Symbol: GABRA4 Gene ID (NCBI): 2557 RRID: AB_2108996 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P48169 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.