Proteintech, 13043-1-AP, MCM4 Polyclonal antibody

Size: 20ul / 150ul The MCM4 (13043-1-AP) by Proteintech is a Polyclonal antibody targeting MCM4 in WB, IHC, IF-P, IP, ELISA applications with reactivity to human samples 13043-1-AP targets MCM4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, HL-60 cells, human liver tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human colon cancer tissue, mouse testis tissue, rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse testis tissue Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of MCM4 protein. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3703 Product name: Recombinant human MCM4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 515-859 aa of BC031061 Sequence: SKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKT Predict reactive species Full Name: minichromosome maintenance complex component 4 Calculated Molecular Weight: 863 aa, 97 kDa Observed Molecular Weight: 97 kDa GenBank Accession Number: BC031061 Gene Symbol: MCM4 Gene ID (NCBI): 4173 RRID: AB_2142293 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P33991 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.