Proteintech, 13124-1-AP, LNX1 Polyclonal antibody

Size: 20ul / 150ul The LNX1 (13124-1-AP) by Proteintech is a Polyclonal antibody targeting LNX1 in WB, ELISA applications with reactivity to human, mouse, rat samples 13124-1-AP targets LNX1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, mouse kidney tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Background Information LNX1 (Ligand of Numb, protein X 1) was originally identified as a binding partner of the cell-fate determinant Numb and was reported to be involved in ubiquitylation and degradation of Numb. LNX1 contains four PDZ domains and may function as a scaffold for a multi-protein complex. LNX1 has two splicing variants, LNX1p70 and LNX1p80. LNX1p70 has the N-terminal Numb binding region and four PDZ domains, whereas LNX1p80 has an additional RING-finger domain at the N-terminus followed by a Numb-binding region and four PDZ domains. This antibody can detect both LNX1p70 and LNX1p80, around 70-80 kDa and 80-100 kDa, respectively. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3823 Product name: Recombinant human LNX1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 378-728 aa of BC022983 Sequence: DSFHVILNKSSPEEQLGIKLVRKVDEPGVFIFNVLDGGVAYRHGQLEENDRVLAINGHDLRYGSPESAAHLIQASERRVHLVVSRQVRQRSPDIFQEAGWNSNGSWSPGPGERSNTPKPLHPTITCHEKVVNIQKDPGESLGMTVAGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVDGVELTEVSRSEAVALLKRTSSSIVLKALEVKEYEPQEDCSSPAALDSNHNMAPPSDWSPSWVMWLELPRCLYNCKDIVLRRNTAGSLGFCIVGGYEEYNGNKPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARLLKELKGRITLTIVSWPGTFL Predict reactive species Full Name: ligand of numb-protein X 1 Calculated Molecular Weight: 728 aa, 81 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC022983 Gene Symbol: LNX1 Gene ID (NCBI): 84708 RRID: AB_2136987 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TBB1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.