Proteintech, 13137-1-AP, MINK1 Polyclonal antibody

Size: 20ul / 150ul The MINK1 (13137-1-AP) by Proteintech is a Polyclonal antibody targeting MINK1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 13137-1-AP targets MINK1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human lymphoma tissue, human skeletal muscle tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Misshapen/Nck-interacting kinase (NIK)-related kinase 1 (MINK1), also known as mitogen-activated protein 4 kinase 6, a serine-threonine protein kinase belonging to the sterile 20 kinase superfamily, has been implicated in diverse physiological and pathophysiological processes, including platelet activation and thrombus formation (PMID: 38723371). MINK1 has the potential to modulate the activation of NLRP3 inflammasome, indicating its pivotal role in governing pyroptosis. Overall, MINK1 is known to regulate cell senescence, cell motility and migration (PMID: 36182968). MINK1 expression has been demonstrated in multiple tissues and organs, including nervous tissue, kidneys, cancer tissues, and immune system cells. (PMID: 39727954) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3795 Product name: Recombinant human MINK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-304 aa of BC034673 Sequence: MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKD Predict reactive species Full Name: misshapen-like kinase 1 (zebrafish) Calculated Molecular Weight: 1332 aa, 150 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC034673 Gene Symbol: MINK1 Gene ID (NCBI): 50488 RRID: AB_10644151 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N4C8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.