Proteintech, 13189-1-AP, RAB25 Polyclonal antibody

Size: 20ul / 150ul The RAB25 (13189-1-AP) by Proteintech is a Polyclonal antibody targeting RAB25 in WB, IHC, ELISA applications with reactivity to human samples 13189-1-AP targets RAB25 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HT-29 cells, Caco-2 cells, T-47D cells Positive IHC detected in: human renal cell carcinoma tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information RAB25 (RAB25, member RAS oncogene family), also known as RAB11C or CATX-8, is a small GTPase belonging to the RAS superfamily of proteins. RAB25 is a key regulator of receptor recycling and polarized vesicular trafficking in epithelial cells. Unlike many RAB proteins, RAB25 expression is predominantly restricted to epithelial tissues and is frequently dysregulated in carcinomas. It functions as a critical determinant of cellular fate through its context-dependent roles in modulating major signaling pathways, including integrin recycling, growth factor receptor trafficking (e.g., EGFR, IGF1R), and autophagy. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3864 Product name: Recombinant human RAB25 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-213 aa of BC033322 Sequence: MGNGTEEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFSHDSRTTIGVEFSTRTVMLGTAAVKAQIWDTAGLERYRAITSAYYRGAVGALLVFDLTKHQTYAVVERWLKELYDHAEATIVVMLVGNKSDLSQAREVPTEEARMFAENNGLLFLETSALDSTNVELAFETVLKEIFAKVSKQRQNSIRTNAITLGSAQAGQEPGPGEKRACCISL Predict reactive species Full Name: RAB25, member RAS oncogene family Calculated Molecular Weight: 213 aa, 23 kDa Observed Molecular Weight: 24 kDa GenBank Accession Number: BC033322 Gene Symbol: RAB25 Gene ID (NCBI): 57111 RRID: AB_2877921 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P57735 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.