Proteintech, 13194-1-AP, FXR1 Polyclonal antibody

Size: 20ul / 150ul The FXR1 (13194-1-AP) by Proteintech is a Polyclonal antibody targeting FXR1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 13194-1-AP targets FXR1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse heart tissue, NIH/3T3 cells Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3873 Product name: Recombinant human FXR1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC028983 Sequence: MAELTVEVRGSNGAFYKGFIKDVHEDSLTVVFENNWQPERQVPFNEVRLPPPPDIKKEISEGDEVEVYSRANDQEPCGWWLAKVRMMKGEFYVIEYAACDATYNEIVTFERLRPVNQNKTVKKNTFFKCTVDVPEDLREACANENAHKDFKKAVGACRIFYHPETTQLMILSASEATVKRVNILSDMHLRSIRTKLMLMSRNEEATKHLECTKQLAAAFHEEFVVREDLMGLAIGTHGSNIQQARKVPGVTAIELDEDTGTFRIYGESADAVKKARGFLEFVEDFIQVPRNLVGKVIGKNGKVIQEIVDKSGVVRVRIEGDNENKLPREDGMVPFVFVGTKESIGNVQVLLEY Predict reactive species Full Name: fragile X mental retardation, autosomal homolog 1 Calculated Molecular Weight: 621 aa, 70 kDa Observed Molecular Weight: 70-80 kDa GenBank Accession Number: BC028983 Gene Symbol: FXR1 Gene ID (NCBI): 8087 RRID: AB_2110702 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P51114 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.