Proteintech, 13209-1-AP, AKR7A3 Polyclonal antibody

Size: 20ul / 150ul The AKR7A3 (13209-1-AP) by Proteintech is a Polyclonal antibody targeting AKR7A3 in WB, IHC, ELISA applications with reactivity to human, mouse samples 13209-1-AP targets AKR7A3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human liver tissue, HepG2 cells, human brain tissue, NIH/3T3 cells Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information AKR7A3 belongs to the aldo‐keto reductase (AKR) superfamily, whose primary role is to reduce aldehydes and ketones to generate primary and secondary alcohols, respectively. These enzymes have been shown to play crucial roles in drug metabolism, carcinogen metabolism, and cellular metabolism. Growing evidence suggests that AKR7A3 can play an essential role in the occurrence of cancers, including breast and liver cancers. (PMID: 36951402) Specification Tested Reactivity: human, mouse Cited Reactivity: rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3991 Product name: Recombinant human AKR7A3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-331 aa of BC025709 Sequence: MSRQLSRARPATVLGAMEMGRRMDAPTSAAVTRAFLERGHTEIDTAFVYSEGQSETILGGLGLRLGGSDCRVKIDTKAIPLFGNSLKPDSLRFQLETSLKRLQCPRVDLFYLHMPDHSTPVEETLRACHQLHQEGKFVELGLSNYAAWEVAEICTLCKSNGWILPTVYQGMYNAITRQVETELFPCLRHFGLRFYAFNPLAGGLLTGKYKYEDKDGKQPVGRFFGNTWAEMYRNRYWKEHHFEGIALVEKALQAAYGASAPSMTSATLRWMYHHSQLQGAHGDAVILGMSSLEQLEQNLAAAEEGPLEPAVVDAFNQAWHLVAHECPNYFR Predict reactive species Full Name: aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase) Calculated Molecular Weight: 331 aa, 37 kDa Observed Molecular Weight: 37 kDa and 55-60 kDa GenBank Accession Number: BC025709 Gene Symbol: AKR7A3 Gene ID (NCBI): 22977 RRID: AB_2224549 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95154 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.