Proteintech, 13216-1-AP, SOX5 Polyclonal antibody

Size: 20ul / 150ul The SOX5 (13216-1-AP) by Proteintech is a Polyclonal antibody targeting SOX5 in WB, IHC, ELISA applications with reactivity to human, mouse samples 13216-1-AP targets SOX5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, SH-SY5Y cells, U-87 MG cells, mouse liver tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Sex determining region Y-box protein 5 (SOX5) is a member of the SOX family, which was identified based on the conserved homology of the high-mobility group DNA-binding motif. It has been reported that SOX5 is involved in the regulation of embryonic development, and is associated with various cancer types, including prostate cancer, glioblastoma, hepatocellular carcinoma, osteosarcoma and nasopharyngeal carcinoma. SOX5 has 5 isoforms with the molecular mass of 42, 71 and 82-84 kDa. (PMID: 30854049) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4092 Product name: Recombinant human SOX5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-376 aa of BC029220 Sequence: DEVAQPLNLSAKPKTSDGKSPTSPTSPHMPALRINSGAGPLKASVPAALASPSARVSTIGYLNDHDAVTKAIQEARQMKEQLRREQQVLDGKVAVVNSLGLNNCRTEKEKTTLESLTQQLAVKQNEEGKFSHAMMDFNLSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCLVDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPGAIAMAGMPSPHLPSEHSSVSSSPEPGMPVIQSTYGVKGEEPHIKEEIQAEDINGEIYDEYDEEEDDPDVDYGSDSENHIAGQAN Predict reactive species Full Name: SRY (sex determining region Y)-box 5 Calculated Molecular Weight: 763 aa, 84 kDa Observed Molecular Weight: 68-70 kDa GenBank Accession Number: BC029220 Gene Symbol: SOX5 Gene ID (NCBI): 6660 RRID: AB_2196089 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35711 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.