Proteintech, 13220-1-AP, GBP5 Polyclonal antibody

Size: 20ul / 150ul The GBP5 (13220-1-AP) by Proteintech is a Polyclonal antibody targeting GBP5 in WB, IHC, IP, ELISA applications with reactivity to human, rat samples 13220-1-AP targets GBP5 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: U-937 cells, Jurkat cells Positive IP detected in: U-937 cells Positive IHC detected in: rat spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa) Specification Tested Reactivity: human, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4103 Product name: Recombinant human GBP5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 382-586 aa of BC031639 Sequence: LETLLDAKQNDICKRNLEASSDYCSALLKDIFGPLEEAVKQGIYSKPGGHNLFIQKTEELKAKYYREPRKGIQAEEVLQKYLKSKESVSHAILQTDQALTETEKKKKEAQVKAEAEKAEAQRLAAIQRQNEQMMQERERLHQEQVRQMEIAKQNWLAEQQKMQEQQMQEQAAQLSTTFQAQNRSLLSELQHAQRTVNNDDPCVLL Predict reactive species Full Name: guanylate binding protein 5 Calculated Molecular Weight: 586 aa, 67 kDa Observed Molecular Weight: 65 kDa, 55 kDa GenBank Accession Number: BC031639 Gene Symbol: GBP5 Gene ID (NCBI): 115362 RRID: AB_2109348 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96PP8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.