Proteintech, 13274-1-AP, CPEB1 Polyclonal antibody

Size: 20ul / 150ul The CPEB1 (13274-1-AP) by Proteintech is a Polyclonal antibody targeting CPEB1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 13274-1-AP targets CPEB1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, HeLa cells, NIH/3T3 cells Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CPEB1, also named as CEBP, CPEB and CPE-BP1, contains two RRM (RNA recognition motif) domains and belongs to the RRM CPEB family. It is a sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. CPEB1 regulated translation is a key process involved in INS signaling. This is a rabbit polyclonal antibody raised against residues near the C terminus of human CPEB1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, asterina pectinifera Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3994 Product name: Recombinant human CPEB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 188-486 aa of BC035348 Sequence: WDLLEAPKDPFSIEREARLHRQAAAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPRCPPKGYVYLVFELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQRLDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEIKTTKFTKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQKNRDSS Predict reactive species Full Name: cytoplasmic polyadenylation element binding protein 1 Calculated Molecular Weight: 486 aa, 54 kDa Observed Molecular Weight: 60-70kDa GenBank Accession Number: BC035348 Gene Symbol: CPEB1 Gene ID (NCBI): 64506 RRID: AB_2083771 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BZB8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.