Proteintech, 13278-1-AP, MX2 Polyclonal antibody

Size: 20ul / 150ul The MX2 (13278-1-AP) by Proteintech is a Polyclonal antibody targeting MX2 in WB, IHC, ELISA applications with reactivity to human samples 13278-1-AP targets MX2 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: THP-1 cells, IFN beta treated THP-1 cells Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Myxovirus resistance 2 (MX2, also known as MxB) is a member of both the dynamin family and the family of large GTPases. MX2 is an interferon-stimulated gene that inhibits the nuclear import of HIV-1 and interacts with the viral capsid and cellular nuclear transport machinery. MX2 mediates the effects of IFN signaling in a cell type and context-dependent manner that can influence cells sensitivity to MAPK pathway targeted therapies. MX2 is also associated with the tumorigenesis process in melanoma(PMID: 33734579). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4020 Product name: Recombinant human MX2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 369-715 aa of BC035293 Sequence: RLAERLTTELIMHIQKSLPLLEGQIRESHQKATEELRRCGADIPSQEADKMFFLIEKIKMFNQDIEKLVEGEEVVRENETRLYNKIREDFKNWVGILATNTQKVKNIIHEEVEKYEKQYRGKELLGFVNYKTFEIIVHQYIQQLVEPALSMLQKAMEIIQQAFINVAKKHFGEFFNLNQTVQSTIEDIKVKHTAKAENMIQLQFRMEQMVFCQDQIYSVVLKKVREEIFNPLGTPSQNMKLNSHFPSNESSVSSFTEIGIHLNAYFLETSKRLANQIPFIIQYFMLRENGDSLQKAMMQILQEKNRYSWLLQEQSETATKRRILKERIYRLTQARHALCQFSSKEIH Predict reactive species Full Name: myxovirus (influenza virus) resistance 2 (mouse) Calculated Molecular Weight: 715 aa, 82 kDa Observed Molecular Weight: 70-82 kDa GenBank Accession Number: BC035293 Gene Symbol: MX2 Gene ID (NCBI): 4600 RRID: AB_3669157 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P20592 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.