Proteintech, 13296-1-AP, IZUMO1 Polyclonal antibody

Size: 20ul / 150ul The IZUMO1 (13296-1-AP) by Proteintech is a Polyclonal antibody targeting IZUMO1 in WB, IF-P, ELISA applications with reactivity to human, mouse samples 13296-1-AP targets IZUMO1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse testis tissue Positive IF-P detected in: mouse testis tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information The Izumo sperm egg fusion gene (IZUMO1) is a crucial member of the immunoglobulin superfamily and plays a crucial role in spermatogenesis, sperm egg interaction, and fusion. The expression of IZUMO1 mRNA was mainly in the testis and sperm and performed an important part in fertilization. IZUMO1 possess a well-conserved N-glycosylation site in the middle of an Ig loop among species. Research indicates that the glycosylated form of IZUMO1 has a molecular weight of 56 kDa, while its dimeric form has a molecular weight of 70 kDa (PMID: 21057513, PMID: 31146500). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4008 Product name: Recombinant human IZUMO1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 31-294 aa of BC034769 Sequence: LALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASRLL Predict reactive species Full Name: izumo sperm-egg fusion 1 Calculated Molecular Weight: 350 aa, 39 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC034769 Gene Symbol: IZUMO1 Gene ID (NCBI): 284359 RRID: AB_2877934 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IYV9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.