Proteintech, 13301-1-AP, Syntaxin 11 Polyclonal antibody

Size: 20ul / 150ul The Syntaxin 11 (13301-1-AP) by Proteintech is a Polyclonal antibody targeting Syntaxin 11 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 13301-1-AP targets Syntaxin 11 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse lung tissue, DU 145 cells, human placenta tissue, human skeletal muscle tissue, Jurkat cells, L02 cells, mouse placenta tissue, rat lung tissue Positive IP detected in: DU 145 cells Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Syntaxin 11 (STX11) belongs to the syntaxin family. It interacts with the SNARE proteins SNAP-23 and VAMP. STX11 behaves as an integral membrane protein despite the lack of a traditional transmembrane domain. The detection of STX11 in human cytotoxic lymphocytes suggests that this protein could play a role in the process leading to degranulation of secretory lysosomes (PMID:17525286). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4097 Product name: Recombinant human STX11 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-287 aa of BC033519 Sequence: MKDRLAELLDLSKQYDQQFPDGDDEFDSPHEDIVFETDHILESLYRDIRDIQDENQLLVADVKRLGKQNARFLTSMRRLSSIKRDTNSIAKAIKARGEVIHCKLRAMKELSEAAEAQHGPHSAVARISRAQYNALTLTFQRAMHDYNQAEMKQRDNCKIRIQRQLEIMGKEVSGDQIEDMFEQGKWDVFSENLLADVKGARAALNEIESRHRELLRLESRIRDVHELFLQMAVLVEKQADTLNVIELNVQKTVDYTGQAKAQVRKAVQYEEKNPCRTLCCFCCPCLK Predict reactive species Full Name: syntaxin 11 Calculated Molecular Weight: 287 aa, 33 kDa Observed Molecular Weight: 33-35 kDa GenBank Accession Number: BC033519 Gene Symbol: Syntaxin 11 Gene ID (NCBI): 8676 RRID: AB_2198214 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75558 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.