Proteintech, 13340-1-AP, PPP3CB Polyclonal antibody

Size: 20ul / 150ul The PPP3CB (13340-1-AP) by Proteintech is a Polyclonal antibody targeting PPP3CB in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13340-1-AP targets PPP3CB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, human heart tissue, HEK-293 cells, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse kidney tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U-251 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4163 Product name: Recombinant human PPP3CB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-311 aa of BC028049 Sequence: MAAPEPARAAPPPPPPPPPPPGADRVVKAVPFPPTHRLTSEEVFDLDGIPRVDVLKNHLVKEGRVDEEIALRIINEGAAILRREKTMIEVEAPITVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWVLKILYPSTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYEACMEAFDSLPLAALLNQQFLCVHGGLSPEIHTLDDIRRLDRFKEPPAFGPMCDLLWSDPSEDFGNEKSQEHFSHNTVRGCSYFYNYPAVCEFLQNNNLLSIIRAHEAQDAGYRMYRKSQTTGFPSL Predict reactive species Full Name: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform Calculated Molecular Weight: 525 aa, 59 kDa Observed Molecular Weight: 59 kDa GenBank Accession Number: BC028049 Gene Symbol: PPP3CB Gene ID (NCBI): 5532 RRID: AB_2284217 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16298 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.