Proteintech, 13345-1-AP, PLA2G4D Polyclonal antibody

Size: 20ul / 150ul The PLA2G4D (13345-1-AP) by Proteintech is a Polyclonal antibody targeting PLA2G4D in WB, IHC, ELISA applications with reactivity to human, mouse samples 13345-1-AP targets PLA2G4D in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, Transfected HEK-293 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Background Information PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4178 Product name: Recombinant human PLA2G4D protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC034571 Sequence: MGRLMRRIPEPRICFLEAIWSNIFSLNLLDAWYDLTSSGESWKQHIKDKTRSLEKEPLTTSGTSSRLEASWLQPGTALAQAFKGFLTGRPLHQRSPNFLQGLQLHQDYCSHKDFSTWADYQLDSMPSQLTPKEPRLCLVDAAYFINTSSPSMFRPGRRLDLILSFDYSLSAPFEVPWSPQGNPSAQPGQAPEASSRATEPLPHTARVPKGRRGVRP Predict reactive species Full Name: phospholipase A2, group IVD (cytosolic) Calculated Molecular Weight: 818 aa, 92 kDa Observed Molecular Weight: 92 kDa GenBank Accession Number: BC034571 Gene Symbol: PLA2G4D Gene ID (NCBI): 283748 RRID: AB_2164607 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86XP0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.