Proteintech, 13361-1-AP, PIP5K3 Polyclonal antibody

Size: 20ul / 150ul The PIP5K3 (13361-1-AP) by Proteintech is a Polyclonal antibody targeting PIP5K3 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13361-1-AP targets PIP5K3 in WB, IHC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse lung tissue, Jurkat cells, mouse brain tissue, rat brain tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PIP5K3 (1-phosphatidylinositol 3-phosphate 5-kinase), also callled PIKFYVE, belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol (PMID: 15902656; 9858586). The content of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. PIKFYVE is the PtdIns(3,5)P2-producing component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PIKFYVE activator VAC14 and the PtdIns(3,5)P2 phosphatase FIG4 (PMID: 17556371). In addition to its phosphoinositide 5-kinase activity, PIKFYVE also has protein kinase activity (PubMed: 11714711; 17556371).PIP5K3 has four isoforms with molecular weights of 237 kDa, 50 kDa, 51 kDa and 62 kDa. This antibody primarily recognizes the 237 kDa isoform. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, pig, sheep Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4177 Product name: Recombinant human PIP5K3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-451 aa of BC032389 Sequence: MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKGRR Predict reactive species Full Name: phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III Calculated Molecular Weight: 451 aa, 50 kDa, 237 kDa Observed Molecular Weight: 237 kDa GenBank Accession Number: BC032389 Gene Symbol: PIP5K3 Gene ID (NCBI): 200576 RRID: AB_10638310 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y2I7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.