Proteintech, 13398-1-AP, GPR161 Polyclonal antibody

Size: 20ul / 150ul The GPR161 (13398-1-AP) by Proteintech is a Polyclonal antibody targeting GPR161 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, canine samples 13398-1-AP targets GPR161 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IF/ICC detected in: MDCK cells, ARPE-19 cells, C2C12 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band probably represents a modified version of GPR161 (PMID: 18250320). Specification Tested Reactivity: human, mouse, canine Cited Reactivity: human, mouse, pig, chicken, zebrafish, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4221 Product name: Recombinant human GPR161 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 321-529 aa of BC028163 Sequence: PLIYGLWNKTVRKELLGMCFGDRYYREPFVQRQRTSRLFSISNRITDLGLSPHLTALMAGGQPLGHSSSTGDTGFSCSQDSGTDMMLLEDYTSDDNPPSHCTCPPKRRSSVTFEDEVEQIKEAAKNSILHVKAEVHKSLDSYAASLAKAIEAEAKINLFGEEALPGVLVTARTVPGGGFGGRRGSRTLVSQRLQLQSIEEGDVLAAEQR Predict reactive species Full Name: G protein-coupled receptor 161 Calculated Molecular Weight: 529 aa, 59 kDa Observed Molecular Weight: 59 kDa GenBank Accession Number: BC028163 Gene Symbol: GPR161 Gene ID (NCBI): 23432 RRID: AB_2113965 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N6U8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.