Proteintech, 13436-1-AP, MTUS1 Polyclonal antibody

Size: 20ul / 150ul The MTUS1 (13436-1-AP) by Proteintech is a Polyclonal antibody targeting MTUS1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13436-1-AP targets MTUS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, K-562 cells, human brain tissue, mouse brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human breast cancer tissue, human thyroid cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information MTUS1, also named as ATBP, ATIP, GK1, KIAA1288 and MTSG1, Belongs to the MTUS1 family. It cooperates with AGTR2 to inhibit ERK2 activation and cell proliferation. MTUS1 may be required for AGTR2 cell surface expression. Together with PTPN6, induces UBE2V2 expression upon angiotensin-II stimulation. Isoform 1 inhibits breast cancer cell proliferation, delays the progression of mitosis by prolonging metaphase and reduces tumor growth. MTUS1 up-regulation during cellular transition from proliferation to quiescence and differentiation. It is a potential tumor suppressor gene located at chromosome 8p21.3.22, near marker D8S254. According to the functional data and intracellular localization, MTUS1 also named as mitochondrial tumor suppressor gene 1 (MTSG1). One main feature common to all ATIP members is the presence of a large C-terminal coiled-coil domain that allows homo- and hetero-dimerization of these proteins. (PMID:12692079, 15123706). The antibody can recognize all the isoforms expect isoform 5(85-90 kDa). The antibody tested HomoDimer isoforms (80kd/110-120/280kd) in Jurkat cell. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4242 Product name: Recombinant human MTUS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC033842 Sequence: MQLQEQFDNLNAAHETSKLEIEASHSEKLELLTKAYEASLSEIKKGHEIEKKSLEDLLSEKQESLEKQINDLKSENDALNEKLKSEEQKRRAREKANLKNPQIMYLEQELESLKAVLEIKNEKLHQQDIKLMKMEKLVDNNTALVDKLKRFQQENEELKARMDKHMAISRQLSTEQAVLQESLEKESKVNKRLSMENEELLWKLHNGDLCSPKRSPTSSAIPLQSPRNSGSFPSPSISPR Predict reactive species Full Name: mitochondrial tumor suppressor 1 Calculated Molecular Weight: 51 kDa, 141 kDa Observed Molecular Weight: 40-48 kDa, 80 kDa, 110-120 kDa, 270-280 kDa GenBank Accession Number: BC033842 Gene Symbol: MTUS1 Gene ID (NCBI): 57509 RRID: AB_10638619 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9ULD2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.