Proteintech, 13484-1-AP, EVL Polyclonal antibody

Size: 20ul / 150ul The EVL (13484-1-AP) by Proteintech is a Polyclonal antibody targeting EVL in WB, IHC, ELISA applications with reactivity to human samples 13484-1-AP targets EVL in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells Positive IHC detected in: human small intestine tissue, human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information EVL (Ena/VASP-like protein) is implicated in processes that requires dynamic actin remodeling such as axon guidance and platelet activation.It has two isoforms, EVL and EVL-I,that are highly expressed in hematopoietic cells of thymus and spleen,and both isoforms can be phosphorylated by PKA in vitro(PMID10945997). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4302 Product name: Recombinant human EVL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-418 aa of BC032358 Sequence: MATSEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTASNTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNIMNSQEGGPSSQRQVQNGPSPDEMDIQRRQVMEQHQQQRQESLERRTSATGPILPPGHPSSAASAPVSCSGPPPPPPPPVPPPPTGATPPPPPPLPAGGAQGSSHDESSMSGLAAAIAGAKLRRVQRPEDASGGSSPSGTSKSDANRASSGGGGGGLMEEMNKLLAKRRKAASQSDKPAEKKEDESQMEDPSTSPSPGTRAASQPPNSSEAGRKPWERSNSVEKPVSSILSRTPSVAKSPEAKSPLQSQPHSRMKPAGSVNDMALDAFDLDRMKQEILEEVVRELHKVKEEIIDAIRQELSGISTT Predict reactive species Full Name: Enah/Vasp-like Calculated Molecular Weight: 418 aa, 45 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC032358 Gene Symbol: EVL Gene ID (NCBI): 51466 RRID: AB_10644121 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UI08 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.