Proteintech, 13572-1-AP, SIRT6 Polyclonal antibody

Size: 20ul / 150ul The SIRT6 (13572-1-AP) by Proteintech is a Polyclonal antibody targeting SIRT6 in WB, IP, ELISA applications with reactivity to human samples 13572-1-AP targets SIRT6 in WB, IHC, IF, IP, ChIP, RIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: BxPC-3 cells, HeLa cells, HepG2 cells, PC-3 cells Positive IP detected in: BxPC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889). This antibody is a rabbit polyclonal antibody raised against full length human SIRT6 antigen. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4469 Product name: Recombinant human SIRT6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC028220 Sequence: MSVNYAAGLSPYADKGKCGLPEIFDPPEELERKVWELARLVWQSSNVVFHTGAGISTASGIPDFRGPHGVWTMEERGLAPKFDTTFESARPTQTHMALVQLERVGLLRFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEECAKCKTQYVRDTVVGTMGLKATGRLCTVAKARGLRACRNADLSITLGTSLQIRPSGNLPLATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPRVLERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPAPHRPPKRVKAKAVPS Predict reactive species Full Name: sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 39-45 kDa GenBank Accession Number: BC028220 Gene Symbol: SIRT6 Gene ID (NCBI): 51548 RRID: AB_2188915 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N6T7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.