Proteintech, 13578-1-AP, ABCG1 Polyclonal antibody

Size: 20ul / 150ul The ABCG1 (13578-1-AP) by Proteintech is a Polyclonal antibody targeting ABCG1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 13578-1-AP targets ABCG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HUVEC cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse placenta tissue, human tonsillitis tissue, human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4479 Product name: Recombinant human ABCG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC029158 Sequence: MACLMAAFSVGTAMNASSYSAEMTEPKSVCVSVDEVVSSNMEATETDLLNGHLKKVDNNLTEAQRFSSLPRRAAVNIEFRDLSYSVPEGPWWRKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMKGAVLINGLPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLSCANTRTGSLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKGLAQGGRSIICTIHQPSAKLFELFDQLYVLSQGQCVYRGKVCNLVPYLRDLGLNCPTYHNPADFVMEVASGEYGDQNSRLVRAVREGMCDSDH Predict reactive species Full Name: ATP-binding cassette, sub-family G (WHITE), member 1 Calculated Molecular Weight: 678 aa, 76 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC029158 Gene Symbol: ABCG1 Gene ID (NCBI): 9619 RRID: AB_2220174 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P45844 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.