Proteintech, 13616-1-AP, HSPA6 Polyclonal antibody

Size: 20ul / 150ul The HSPA6 (13616-1-AP) by Proteintech is a Polyclonal antibody targeting HSPA6 in WB, IHC, ELISA applications with reactivity to human samples 13616-1-AP targets HSPA6 in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HL-60 cells, MCF-7 cells, U-937 cells Positive IHC detected in: mouse lung tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458) Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4522 Product name: Recombinant human HSPA6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 294-643 aa of BC035665 Sequence: DFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDVVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAVLMGDKCEKVQDLLLLDVAPLSLGLETAGGVMTTLIQRNATIPTKQTQTFTTYSDNQPGVFIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILSVTATDRSTGKANKITITNDKGRLSKEEVERMVHEAEQYKAEDEAQRDRVAAKNSLEAHVFHVKGSLQEESLRDKIPEEDRRKMQDKCREVLAWLEHNQLAEKEEYEHQKRELEQICRPIFSRLYGGPGVPGGSSCGTQARQGDPSTGPIIEEVD Predict reactive species Full Name: heat shock 70kDa protein 6 (HSP70B') Calculated Molecular Weight: 643 aa, 71 kDa Observed Molecular Weight: 68-71 kDa GenBank Accession Number: BC035665 Gene Symbol: HSPA6 Gene ID (NCBI): 3310 RRID: AB_2120122 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P17066 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.