Proteintech, 13642-1-AP, UIMC1 Polyclonal antibody

Size: 20ul / 150ul The UIMC1 (13642-1-AP) by Proteintech is a Polyclonal antibody targeting UIMC1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 13642-1-AP targets UIMC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse colon tissue, COLO 320 cells, HeLa cells, Raji cells Positive IP detected in: COLO 320 cells Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Hela cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4582 Product name: Recombinant human UIMC1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 202-553 aa of BC032561 Sequence: DQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQSSQGIVEETSEEGNSVPASQSVAALTSKRSLVLMPESSAEEITVCPETQLSSSETFDLEREVSPGSRDILDGVRIIMADKEVGNKEDAEKEVAISTFSSSNQVSCPLCDQCFPPTKIERHAMYCNGLMEEDTVLTRRQKEAKTKSDSGTAAQTSLDIDKNEKCYLCKSLVPFREYQCHVDSCLQLAKADQGDGPEGSGRACSTVEGKWQQRLKNPKEKGHSEGRLLSFLEQSEHKTSDADIKSSETGAFRVPSPGMEEAGCSREMQSSFTRRDLNESPVKSFVSISEATDCLVDFKKQVTVQPGSRTRTKAGRGRRRKF Predict reactive species Full Name: ubiquitin interaction motif containing 1 Calculated Molecular Weight: 719 aa, 80 kDa Observed Molecular Weight: 97 kDa, 80 kDa, 71-75 kDa, 61 kDa GenBank Accession Number: BC032561 Gene Symbol: UIMC1 Gene ID (NCBI): 51720 RRID: AB_10641052 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96RL1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.