Proteintech, 13662-1-AP, LTA4H Polyclonal antibody

Size: 20ul / 150ul The LTA4H (13662-1-AP) by Proteintech is a Polyclonal antibody targeting LTA4H in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 13662-1-AP targets LTA4H in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, Jurkat cells, HeLa cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human skin tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information LTA4H(Leukotriene A-4 hydrolase), also named as LTA4, is essential for the conversion of LTA4 to LTB4, an inflammatory lipid mediator(PMID:10601658). LTA4H also possesses aminopeptidase activity that it can degrade PGP, which is a biomarker for chronic obstructive pulmonary disease and is implicated in neutrophil persistence in the lung(PMID:20813919). This protein has 4 isoforms produced by alternative splicing with the MW of 69, 59, 57, 67 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4568 Product name: Recombinant human LTA4H protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 260-611 aa of BC032528 Sequence: LVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGELQNSVKTFGETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVDWNAWLYSPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQLNEFLAQTLQRAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEDAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAVRTYQEHKASMHPVTAMLVGKDLKVD Predict reactive species Full Name: leukotriene A4 hydrolase Calculated Molecular Weight: 611 aa, 69 kDa Observed Molecular Weight: 57-70 kDa GenBank Accession Number: BC032528 Gene Symbol: LTA4H Gene ID (NCBI): 4048 RRID: AB_2138747 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P09960 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.