Proteintech, 13663-1-AP, TRIM17 Polyclonal antibody

Size: 20ul / 150ul The TRIM17 (13663-1-AP) by Proteintech is a Polyclonal antibody targeting TRIM17 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13663-1-AP targets TRIM17 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, COLO 320 cells, mouse spleen tissue, mouse thymus tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TRIM17 (Tripartite motif-containing 17) also known as RNF16 (Ring Finger protein 16) or terf (testis RING finger protein), is a member of the TRIM family, a large class of RING-containing E3 ubiquitin-ligases. It is expressed at low levels in adult tissues, except in testis and in some brain regions (PMID: 34069831). TRIM17 contributes to gastric cancer survival through regulating BAX protein stability and antagonizing apoptosis, which provides a promising therapeutic target for GC treatment and a biomarker for prognosis (PMID: 27562068, 37697039). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4572 Product name: Recombinant human TRIM17 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 177-477 aa of BC033788 Sequence: KVKERRERIVLEFEKMNLYLVEEEQRLLQALETEEEETASRLRESVACLDRQGHSLELLLLQLEERSTQGPLQMLQDMKEPLSRKNNVSVQCPEVAPPTRPRTVCRVPGQIEVLRGFLEDVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSKDRFVAYPCAVGQTAFSSGRHYWEVGMNITGDALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQPFFCLGAPKSGQMVISTVTMWVKG Predict reactive species Full Name: tripartite motif-containing 17 Calculated Molecular Weight: 477 aa, 54 kDa Observed Molecular Weight: 54 kDa GenBank Accession Number: BC033788 Gene Symbol: TRIM17 Gene ID (NCBI): 51127 RRID: AB_10644443 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y577 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.