Proteintech, 13728-1-AP, KERA Polyclonal antibody

Size: 20ul / 150ul The KERA (13728-1-AP) by Proteintech is a Polyclonal antibody targeting KERA in WB, ELISA applications with reactivity to human samples 13728-1-AP targets KERA in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U-251 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information Keratocan and Lumican are the predominant class II SLRPs(Small leucine-rich proteoglycans) in the corneal stroma. While not totally corneal-specific together these SLRPs are excellent biomarkers for the tissue. Mutant mice deficient for keratocan expression have transparent but thin corneal stromas with a mild increase in collagen fibril diameter with no further abnormalities.In humans, mutations in the keratocan gene are associated with a flattened cornea in a rare condition known as cornea plana. The relative specificity of keratocan to corneal keratocytes has been useful for tissue-specific targeting for genetic manipulation of the corneal stroma.(PMID: 32663498) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4689 Product name: Recombinant human KERA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 24-331 aa of BC032667 Sequence: RQVYEVHDSDDWTIHDFECPMECFCPPSFPTALYCENRGLKEIPAIPSRIWYLYLQNNLIETIPEKPFENATQLRWINLNKNKITNYGIEKGALSQLKKLLFLFLEDNELEEVPSPLPRSLEQLQLARNKVSRIPQGTFSNLENLTLLDLQNNKLVDNAFQRDTFKGLKNLMQLNMAKNALRNMPPRLPANTMQLFLDNNSIEGIPENYFNVIPKVAFLRLNHNKLSDEGLPSRGFDVSSILDLQLSHNQLTKVPRISAHLQHLHLDHNKIKSVNVSVICPSPSMLPAERDSFSYGPHLRYLRLDGNE Predict reactive species Full Name: keratocan Calculated Molecular Weight: 352 aa, 41 kDa Observed Molecular Weight: 40-50 kDa GenBank Accession Number: BC032667 Gene Symbol: KERA Gene ID (NCBI): 11081 RRID: AB_3669169 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60938 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.