Proteintech, 13766-1-AP, Entactin Polyclonal antibody

Size: 20ul / 150ul The Entactin (13766-1-AP) by Proteintech is a Polyclonal antibody targeting Entactin in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13766-1-AP targets Entactin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human placenta tissue, mouse heart tissue, mouse placenta tissue Positive IP detected in: human placenta tissue Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Nidogen 1 (Entactin), first identified from a basement membrane-secreting cell line and the murine EHS tumor, is together with perlecan, laminin, and collagen IV, a ubiquitous component of basement membranes (PMID: 19530222). Nidogen-1 is made by mesenchymal cells and is incorporated into the epithelial BM. It is a sulfated glycoprotein of 150 kDa with three globular domains, G1, G2 and G3, and contains binding sites for other ECM molecules (PMID: 10958695, 10671374). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4749 Product name: Recombinant human NID1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 28-400 aa of BC045606 Sequence: CLSRQELFPFGPGQGDLELEDGDDFVSPALELRGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESIENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVH Predict reactive species Full Name: nidogen 1 Calculated Molecular Weight: 1247 aa, 136 kDa Observed Molecular Weight: 136-150 kDa GenBank Accession Number: BC045606 Gene Symbol: Entactin/NID Gene ID (NCBI): 4811 RRID: AB_10642564 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P14543 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.