Proteintech, 13847-1-AP, ACSL6 Polyclonal antibody

Size: 20ul / 150ul The ACSL6 (13847-1-AP) by Proteintech is a Polyclonal antibody targeting ACSL6 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 13847-1-AP targets ACSL6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human pancreas cancer tissue, human urothelial carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information ACSL6 (Acyl-CoA Synthetase Long Chain Family Member 6) is a long-chain lipoyl-coenzyme A synthetase that is mainly responsible for catalyzing the binding of long-chain fatty acids to coenzyme A to form acyl-coenzyme A (Acyl-CoA). ACSL6 is involved in fatty acid metabolic transporter and the synthesis of cell membrane phospholipids, which affects the cell structure and function. ACSL6 plays an extremely important role in the pathogenesis of cancers such as hepatocellular carcinoma, lung cancer and neuroblastoma, suggesting that it may serve as a biomarker and therapeutic target for cancer therapy. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4817 Product name: Recombinant human ACSL6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 48-349 aa of BC047453 Sequence: THYYDDARTMYQVFRRGLSISGNGPCLGFRKPKQPYQWLSYQEVADRAEFLGSGLLQHNCKACTDQFIGVFAQNRPEWIIVELACYTYSMVVVPLYDTLGPGAIRYIINTADISTVIVDKPQKAVLLLEHVERKETPGLKLIILMDPFEEALKERGQKCGVVIKSMQAVEDCGQANHQAPVPPQPDDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTEGDIRLLSDDMKALCPTIFPVVPRLLNRMYDKIFSQANTPLKRWLLEFAAKRKQAEVRSGIIRNDSIWDELFFNKIQASL Predict reactive species Full Name: acyl-CoA synthetase long-chain family member 6 Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 74 kDa GenBank Accession Number: BC047453 Gene Symbol: ACSL6 Gene ID (NCBI): 23305 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UKU0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.