Proteintech, 13913-1-AP, SODD/BAG4 Polyclonal antibody

Size: 20ul / 150ul The SODD/BAG4 (13913-1-AP) by Proteintech is a Polyclonal antibody targeting SODD/BAG4 in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 13913-1-AP targets SODD/BAG4 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, MCF-7 cells Positive IP detected in: K-562 cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861). Specification Tested Reactivity: human Cited Reactivity: human, artemia sinica Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4905 Product name: Recombinant human BAG4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 107-457 aa of BC038505 Sequence: ARSRAPYPSTYPVRPELQGQSLNSYTNGAYGPTYPPGPGANTASYSGAYYAPGYTQTSYSTEVPSTYRSSGNSPTPVSRWIYPQQDCQTEAPPLRGQVPGYPPSQNPGMTLPHYPYGDGNRSVPQSGPTVRPQEDAWASPGAYGMGGRYPWPSSAPSAPPGNLYMTESTSPWPSSGSPQSPPSPPVQQPKDSSYPYSQSDQSMNRHNFPCSVHQYESSGTVNNDDSDLLDSQVQYSAEPQLYGNATSDHPNNQDQSSSLPEECVPSDESTPPSIKKIIHVLEKVQYLEQEVEEFVGKKTDKAYWLLEEMLTKELLELDSVETGGQDSVRQARKEAVCKIQAILEKLEKKGL Predict reactive species Full Name: BCL2-associated athanogene 4 Calculated Molecular Weight: 457 aa, 50 kDa Observed Molecular Weight: 60 kDa, 66-70 kDa GenBank Accession Number: BC038505 Gene Symbol: SODD/BAG4 Gene ID (NCBI): 9530 RRID: AB_2243308 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95429 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.