Proteintech, 13940-1-AP, GABA B receptor 1 Polyclonal antibody

Size: 20ul / 150ul The GABA B receptor 1 (13940-1-AP) by Proteintech is a Polyclonal antibody targeting GABA B receptor 1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 13940-1-AP targets GABA B receptor 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, 37°C incubated mouse brain tissue, rat brain tissue, 37°C incubated rat brain tissue Positive IHC detected in: mouse brain tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information GABA B receptor 1 (GABBR1) is an essential protein subunit that forms the functional GABA B receptor, a metabotropic receptor for the primary inhibitory neurotransmitter in the central nervous system, gamma-aminobutyric acid (GABA). Unlike the fast-acting ionotropic GABA A receptors, GABA B receptors mediate slow, prolonged inhibitory signaling through G-proteins. Two bands with the apparent molecular weights of 130 and 105 kDa were corresponding to GABAB1a and GABAB1b proteins, were identified in the brain extract and membrane extract of wild-type mice (PMID: 12970075). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4838 Product name: Recombinant human GABBR1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 101-471 aa of BC041332 Sequence: SKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAARMWNLIVLSYGSSSPALSNRQRFPTFFRTHPSATLHNPTRVKLFEKWGWKKIATIQQTTEVFTSTLDDLEERVKEAGIEITFRQSFFSDPAVPVKNLKRQDARIIVGLFYETEARKVFCEVYKERLFGKKYVWFLIGWYADNWFKIYDPSINCTVDEMTEAVEGHITTEIVMLNPANTRSISNMTSQEFVEKLTKRLKRHPEETGGFQEAPLAYDAIWALALALNKTSGGGGRSGVRLEDFNYNNQTITDQIYRAMNSSSFEGVSGHVVFDASGSRMAWTLIEQLQGGSYKKIGYYDSTKDDLSWSKTDKWIGGSPPADQTLVIKTFRFLS Predict reactive species Full Name: gamma-aminobutyric acid (GABA) B receptor, 1 Calculated Molecular Weight: 961 aa, 108 kDa Observed Molecular Weight: 105-130 kDa GenBank Accession Number: BC041332 Gene Symbol: GABBR1 Gene ID (NCBI): 2550 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UBS5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.