Proteintech, 13965-1-AP, MICAL2 Polyclonal antibody

Size: 20ul / 150ul The MICAL2 (13965-1-AP) by Proteintech is a Polyclonal antibody targeting MICAL2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 13965-1-AP targets MICAL2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: DU 145 cells, PC-3 cells, U-251 cells, U-87 MG cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions. There are three known isoforms, MICAL1, MICAL2, and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, and a Lin11, Isl-1, and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variant of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4950 Product name: Recombinant human MICAL2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 771-1124 aa of BC044577 Sequence: KAAHLASMFGHGDFPQNKLLSKGLSHTHPPSPPSRLPSPDPAASSSPSTVDSASPARKEKKSPSGFHFHPSHLRTVHPQLTVGKVSSGIGAAAEVLVNLYMNDHRPKAQATSPDLESMRKSFPLNLGGSDTCYFCKKRVYVMERLSAEGHFFHRECFRCSICATTLRLAAYTFDCDEGKFYCKPHFIHCKTNSKQRKRRAELKQQREEEATWQEQEAPRRDTPTESSCAVAAIGTLEGSPPVHFSLPVLHPLLG Predict reactive species Full Name: microtubule associated monoxygenase, calponin and LIM domain containing 2 Calculated Molecular Weight: 127 kDa Observed Molecular Weight: 95 kDa, 112 kDa GenBank Accession Number: BC044577 Gene Symbol: MICAL2 Gene ID (NCBI): 9645 RRID: AB_2143764 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O94851 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.