Proteintech, 13974-1-AP, CTNNA3 Polyclonal antibody

Size: 20ul / 150ul The CTNNA3 (13974-1-AP) by Proteintech is a Polyclonal antibody targeting CTNNA3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 13974-1-AP targets CTNNA3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, HEK-293 Positive IP detected in: mouse testis tissue Positive IHC detected in: human liver cancer tissue, human heart tissue, human placenta tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:2000 Background Information Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. The alpha catenin family of proteins consists of three members, alpha-E-catenin (CTNNA1), alpha-N-catenin (CTNNA2), and alpha-T-catenin (CTNNA3)(PMID: 21598020). CTNNA3 is a cell adhesion molecule. In intercalated discs of the heart, CTNNA3 is a component of a unique hybrid adhering junction, or area composita, that contains proteins associated with both desmosomes and adherens junctions (PMID: 22421363). Mutations in CTNNA3 are associated with arrhythmogenic right ventricular dysplasia, familial 13(PMID: 23136403). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5008 Product name: Recombinant human CTNNA3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC030646 Sequence: MSAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPSSRKKGRSKRASVLLASVEEATWNLLDKGEKIAQEATVLKDELTASLEKVRKESEALKVSAERFTDDPCFLPKREAVVQAARALLAAVTRLLILADMIDVMCLLQHVSAFQRTFESLKNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARASLKENSPLLHSICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSALDELENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAIRQALQDLLSEYMNNLCNQKSDLGLTGVKIKLLEECIIFWSL Predict reactive species Full Name: catenin (cadherin-associated protein), alpha 3 Calculated Molecular Weight: 100 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC030646 Gene Symbol: CTNNA3 Gene ID (NCBI): 29119 RRID: AB_2088078 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UI47 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.