Proteintech, 13996-1-AP, PARD6B Polyclonal antibody

Size: 20ul / 150ul The PARD6B (13996-1-AP) by Proteintech is a Polyclonal antibody targeting PARD6B in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 13996-1-AP targets PARD6B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, human placenta tissue, PC-3 cells, LNCaP cells Positive IP detected in: HEK-293 cells Positive IHC detected in: mouse kidney tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information PARD6B (also named PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and fetal kidneys, while much weaker but significant signals were observed in the placenta, lung, and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5083 Product name: Recombinant human PARD6B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-372 aa of BC060847 Sequence: MNRSHRHGAGSGCLGTMEVKSKFGAEFRRFSLERSKPGKFEEFYGLLQHVHKIPNVDVLVGYADIHGDLLPINNDDNYHKAVSTANPLLRIFIQKKEEADYSAFGTDTLIKKKNVLTNVLRPDNHRKKPHIVISMPQDFRPVSSIIDVDILPETHRRVRLYKYGTEKPLGFYIRDGSSVRVTPHGLEKVPGIFISRLVPGGLAQSTGLLAVNDEVLEVNGIEVSGKSLDQVTDMMIANSRNLIITVRPANQRNNVVRNSRTSGSSGQSTDNSLLGYPQQIEPSFEPEDEDSEEDDIIIEDNGVPQQIPKAVPNTESLESLTQIELSFESGQNGFIPSNEVSLAAIASSSNTEFETHAPDQKLLEEDGTIITL Predict reactive species Full Name: par-6 partitioning defective 6 homolog beta (C. elegans) Calculated Molecular Weight: 41 kDa Observed Molecular Weight: 50-53 kDa GenBank Accession Number: BC060847 Gene Symbol: PARD6B Gene ID (NCBI): 84612 RRID: AB_2159779 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BYG5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.