Proteintech, 14018-1-AP, BACH1 Polyclonal antibody

Size: 20ul / 150ul The BACH1 (14018-1-AP) by Proteintech is a Polyclonal antibody targeting BACH1 in WB, IHC, ELISA applications with reactivity to human samples 14018-1-AP targets BACH1 in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, MCF-7 cells, Raji cells, HeLa cells, Jurkat cells, K-562 cells Positive IHC detected in: human cervical cancer tissue, human breast hyperplasia tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:750-1:3000 Background Information BTB and CNC homology 1 (Bach1) isa transcription factor expressed in many tissues, with key roles in cell cycleregulation in homeostasis and in cancer in disease.What is the molecular weight of BACH1?Bach1 consists of736 amino acids and is amember of the cap'n'collar type of basic region leucine zipper factorfamily (CNC-bZip) with several atypical protein domains.Although its predicted MW is 82 kDa, it runsanomalouslyat100-110 kDa in SDS-PAGE.What is the cellular localization of BACH1?As it is a transcription factor, BACH1 is found in the nucleus of a number of mammalian cells, where it forms aheterodimer with small Maf proteins. Once it has formed part of a heterodimer, BACH1 is able to bind to theMafrecognition elements (MAREs)of target genes. During oxidative stress,BACH1 can be inactivated either byhemeor by cadmium, at which point it is exported from the nucleus into the cytoplasm, where it localizes tomicrotubules(PMID: 15809329).What is the function of BACH1?BACH1 regulates the production of reactive oxygen species, with many of its downstream targets such as hemeoxygenase-1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1) (PMID: 12356737; PMID: 15734732) involvedin theoxidative stress response. When the cell undergoes oxidative stress, BACH1 is displaced bynuclear factor(erythroid-derived2)-like-2 (Nrf2), as both compete in binding to the MAREs in target genes. BACH1 also plays arolein cellproliferation and survival, as it regulates several cell cycle genes such as cyclin-dependent kinase 6(CDK6) andBCL2-like 11 (BCL2L11).What is the role of BACH1 in disease?BACH1 has been described in a number of cancer types, including breast, prostate, and melanoma. The functionofBACH1 appears varied in these tissues, with evidence that it may both promote and repress metastasis,influenceepigenetics, and regulate the growth of cancer cells (PMID: 22875853; PMID: 28889753; PMID: 26787892).BACH1may act on the same gene in different contexts to repress or activate it (PMID: 8887638). Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5128 Product name: Recombinant human BACH1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 387-736 aa of BC063307 Sequence: SSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLLKERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSFLISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQMSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE Predict reactive species Full Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1 Calculated Molecular Weight: 82 kDa Observed Molecular Weight: 100-110 kDa GenBank Accession Number: BC063307 Gene Symbol: BACH1 Gene ID (NCBI): 571 ENSEMBL Gene ID: ENSG00000156273 RRID: AB_2274498 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14867 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.