Proteintech, 14026-1-AP, ZEB2 Polyclonal antibody

Size: 20ul / 150ul The ZEB2 (14026-1-AP) by Proteintech is a Polyclonal antibody targeting ZEB2 in WB, IHC, ELISA applications with reactivity to human samples 14026-1-AP targets ZEB2 in WB, IHC, IF, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HepG2 cells, HeLa cells Positive IHC detected in: human gliomas tissue, human lung cancer tissue, human renal cell carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 ) Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, chicken, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5171 Product name: Recombinant human ZEB2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC060819 Sequence: MKQPIMADGPRCKRRKQANPRRKNVVNYDNVVDTGSETDEEDKLHIAEDDGIANPLDQETSPASVPNHESSPHVSQALLPREEEEDEIREGGVEHPWHNNEILQASVDGPGKCLKSLDLFLYHLHIFN Predict reactive species Full Name: zinc finger E-box binding homeobox 2 Calculated Molecular Weight: 136 kDa Observed Molecular Weight: 150-170 kDa GenBank Accession Number: BC060819 Gene Symbol: ZEB2 Gene ID (NCBI): 9839 RRID: AB_2878001 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60315 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.