Proteintech, 14049-1-AP, MAN1A2 Polyclonal antibody

Size: 20ul / 150ul The MAN1A2 (14049-1-AP) by Proteintech is a Polyclonal antibody targeting MAN1A2 in WB, IP, IHC, ELISA applications with reactivity to human samples 14049-1-AP targets MAN1A2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human placenta tissue Positive IP detected in: human placenta tissue Positive IHC detected in: human placenta tissue, human heart tissue, human lung tissue, human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information The first group or Class I mannosidase is comprised of α1-2 mannosidases from mammals and yeast with encoded polypeptides of 65-75 kDa. Most of them are Ca2+-dependent enzymes located in the ER and/or Golgi which are inhibited by substrate mimics like kifunensine and deoxymannojirimycin. Their specificity is restricted to α(1-2) linkages, and they are mostly inactive towards artificial substrates like arylmannosides(PMID:10207175). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5240 Product name: Recombinant human MAN1A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 292-641 aa of BC063300 Sequence: VQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKNGHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPLPVLHLANTTLSGNPAVR Predict reactive species Full Name: mannosidase, alpha, class 1A, member 2 Calculated Molecular Weight: 641 aa, 73 kDa Observed Molecular Weight: 73 kDa GenBank Accession Number: BC063300 Gene Symbol: MAN1A2 Gene ID (NCBI): 10905 RRID: AB_2266033 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60476 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.