Proteintech, 14056-1-AP, RGS7 Polyclonal antibody

Size: 20ul / 150ul The RGS7 (14056-1-AP) by Proteintech is a Polyclonal antibody targeting RGS7 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 14056-1-AP targets RGS7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SH-SY5Y cells Positive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Regulator of G-protein signaling 7 (RGS7) expression is both necessary and sufficient to promote doxorubicin-dependent acetylation and activation of NF-κB subunit p65 and resultant production of pro-inflammatory cytokines from cardiomyocytes (PMID: 37589751). RGS7 promotes apoptosis in cardiac myocytes by increasing oxidative stress and impairing mitochondrial function, actions that involve a direct and functional interaction between RGS7 and the Ca2+/calmodulin-dependent kinase CaMKII (PMID: 36574707). In non-neuronal cell types, it is possible that a greater proportion of RGS7 may be present in the cytosol where it is free to interact with additional effectors (PMID: 36574707). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag4982 Product name: Recombinant human RGS7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 139-487 aa of BC022009 Sequence: NKARLELADYEAESLARLQRAFARKWEFIFMQAEAQAKVDKKRDKIERKILDSQERAFWDVHRPVPGCVNTTEVDIKKSSRMRNPHKTRKSVYGLQNDIRSHSPTHTPTPETKPPTEDELQQQIKYWQIQLDRHRLKMSKVADSLLSYTEQYLEYDPFLLPPDPSNPWLSDDTTFWELEASKEPSQQRVKRWGFGMDEALKDPVGREQFLKFLESEFSSENLRFWLAVEDLKKRPIKEVPSRVQEIWQEFLAPGAPSAINLDSKSYDKTTHNVKEPGRYTFEDAQEHIYKLMKSDSYPRFIRSSAYQELLQAKKKSGNSMDRRTSFEKFAQNVGKSLTSKRLTSLAQSY Predict reactive species Full Name: regulator of G-protein signaling 7 Calculated Molecular Weight: 58 kDa Observed Molecular Weight: 58-62 kDa GenBank Accession Number: BC022009 Gene Symbol: RGS7 Gene ID (NCBI): 6000 RRID: AB_2238316 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P49802 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.