Proteintech, 14067-1-AP, CCP1 Polyclonal antibody

Size: 20ul / 150ul The CCP1 (14067-1-AP) by Proteintech is a Polyclonal antibody targeting CCP1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 14067-1-AP targets CCP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, A549 cells, mouse testis tissue Positive IP detected in: mouse testis tissue Positive IHC detected in: human testis tissue, human heart tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information AGTPBP1, also known as CCP1 (cytosolic carboxy-peptidase 1), or Nna1, belongs to the peptidase M14 family. AGTPBP1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motiff. Thus, it may contribute to nuclear signaling events in differentiating and regenerating neurons. It serves important functions in the nervous system, and AGTPBP1 mutations may cause Purkinje cell degeneration (pcd) phenotypes. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5122 Product name: Recombinant human CCP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 837-1186 aa of BC060815 Sequence: VLCETLSGNSCPLVTITAMPESNYYEHICHFRNRPYVFLSARVHPGETNASWVMKGTLEYLMSNNPTAQSLRESYIFKIVPMLNPDGVINGNHRCSLSGEDLNRQWQSPSPDLHPTIYHAKGLLQYLAAVKRLPLVYCDYHGHSRKKNVFMYGCSIKETVWHTNDNATSCDVVEDTGYRTLPKILSHIAPAFCMSSCSFVVEKSKESTARVVVWREIGVQRSYTMESTLCGCDQGKYKGLQIGTRELEEMGAKFCVGLLRLKRLTSPLEYNLPSSLLDFENDLIESSCKVTSPTTYVLDEDEPRFLEEVDYSAESNDELDIELAENVGDYEPSAQEEVLSDSELSRTYLP Predict reactive species Full Name: ATP/GTP binding protein 1 Calculated Molecular Weight: 138 kDa Observed Molecular Weight: 138 kDa GenBank Accession Number: BC060815 Gene Symbol: CCP1 Gene ID (NCBI): 23287 RRID: AB_2225559 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UPW5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.