Proteintech, 14075-1-AP, PDGF-D Polyclonal antibody

Size: 20ul / 150ul The PDGF-D (14075-1-AP) by Proteintech is a Polyclonal antibody targeting PDGF-D in IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, rat samples 14075-1-AP targets PDGF-D in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive IHC detected in: human colon cancer tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: rat brain tissue Positive IF/ICC detected in: A549 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Platelet-derived growth factor-D (PDGF-D) is the newest member of the PDGF family. It is secreted as a latent dimer that must undergo extracellular proteolytic removal of its N-terminal CUB domain before the growth-factor domain can activate the β-PDGFR. Once activated, PDGF-D drives proliferation, migration and survival of mesenchymal cells and promotes angiogenesis, wound healing and tissue remodeling. Clinically, PDGF-D is up-regulated in several cancers and in fibrotic diseases of the heart, kidney and lung, making it a potential therapeutic target. Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse, rat, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5154 Product name: Recombinant human PDGFD protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-364 aa of BC030645 Sequence: MHRLIFVYTLICANFCSCRDTSATPQSASIKALRNANLRRDDLYRRDETIQVKGNGYVQSPRFPNSYPRNLLLTWRLHSQENTRIQLVFDNQFGLEEAENDICRYDFVEVEDISETSTIIRGRWCGHKEVPPRIKSRTNQIKITFKSDDYFVAKPGFKIYYSLLEDFQPAAASETNWESVTSSISGVSYNSPSVTDPTLIADALDKKIAEFDTVEDLLKYFNPESWQEDLENMYLDTPRYRGRSYHDRKSKVDLDRLNDDAKRYSCTPRNYSVNIREELKLANVVFFPRCLLVQRCGGNCGCGTVNWRSCTCNSGKTVKKYHEVLQFEPGHIKRRGRAKTMALVDIQLDHHERCDCICSSRPPR Predict reactive species Full Name: platelet derived growth factor D Calculated Molecular Weight: 43 kDa GenBank Accession Number: BC030645 Gene Symbol: PDGFD Gene ID (NCBI): 80310 RRID: AB_2162208 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9GZP0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.