Proteintech, 14092-1-AP, ARHGEF7 Polyclonal antibody

Size: 20ul / 150ul The ARHGEF7 (14092-1-AP) by Proteintech is a Polyclonal antibody targeting ARHGEF7 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14092-1-AP targets ARHGEF7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-12 cells, mouse skeletal muscle tissue, K-562 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, Transfected HEK-293 cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse brain tissue, human rectal cancer tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5223 Product name: Recombinant human ARHGEF7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 455-803 aa of BC060776 Sequence: KELELQILTEAIRNWEGDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADDDQPSLDSLGRRSSLSRLEPSDLSEDSDYDSIWTAHSYRMGSTSRKSCCSYISHQN Predict reactive species Full Name: Rho guanine nucleotide exchange factor (GEF) 7 Calculated Molecular Weight: 803 aa, 90 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC060776 Gene Symbol: ARHGEF7 Gene ID (NCBI): 8874 RRID: AB_2227567 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14155 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.