Proteintech, 14168-1-AP, Talin-1 Polyclonal antibody

Size: 20ul / 150ul The Talin-1 (14168-1-AP) by Proteintech is a Polyclonal antibody targeting Talin-1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 14168-1-AP targets Talin-1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HUVEC cells, HeLa cells, NIH/3T3 cells, C6 cells, HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: human liver cancer tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HUVEC cells, HeLa cells, HepG2 cells, A549 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, chicken Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5367 Product name: Recombinant human TLN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2193-2541 aa of BC042923 Sequence: GNSCRQEDVIATANLSRRAIADMLRACKEAAYHPEVAPDVRLRALHYGRECANGYLELLDHVLLTLQKPSPELKQQLTGHSKRVAGSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANALDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASQEKLISSAKQVAASTAQLLVACKVKADQDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFEEQENETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELRDEH Predict reactive species Full Name: talin 1 Calculated Molecular Weight: 2541 aa, 270 kDa Observed Molecular Weight: 250-270 kDa GenBank Accession Number: BC042923 Gene Symbol: Talin-1 Gene ID (NCBI): 7094 RRID: AB_2271861 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y490 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.