Proteintech, 14181-1-AP, Alpha 1B-Glycoprotein/A1BG Polyclonal antibody

Size: 20ul / 150ul The Alpha 1B-Glycoprotein/A1BG (14181-1-AP) by Proteintech is a Polyclonal antibody targeting Alpha 1B-Glycoprotein/A1BG in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 14181-1-AP targets Alpha 1B-Glycoprotein/A1BG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human plasma tissue, BxPC-3 cells, MCF-7 cells, mouse kidney tissue Positive IHC detected in: human hepatocirrhosis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:20000 Immunohistochemistry (IHC): IHC : 1:2500-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5414 Product name: Recombinant human A1BG protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-373 aa of BC035719 Sequence: MAPVSWITPGLKTTAVCRGVLRGVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGNYSCSYRTDGEGALSEPSATVTIEELAAPPPPVLMHHGESSQVLHPGNKVTLTCVAPLSGVDFQLRRGEKELLVPRSSTSPDRIFFHLNAVALGDGGHYTCRYRLHDNQNGWSGDSAPVELILSDETLPAPEFSPEPESGRALRLRCLAPLEGARFALVREDRGGRRVHRFQSPAGTEALFELHNISVADSANYSCVYVDLKPPFGGSAPSERLELHVDGPPPRPQLRATWSGAVLAGRDAVLRCEGPIPDVTFELLREGETKAVKTVRTPGAAANLELIFVGPQHAGNYRCRYRSWVPHTFESELSDPVELLVAES Predict reactive species Full Name: alpha-1-B glycoprotein Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC035719 Gene Symbol: A1BG Gene ID (NCBI): 1 RRID: AB_10640441 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04217 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.