Proteintech, 14261-1-AP, PNPLA6 Polyclonal antibody

Size: 20ul / 150ul The PNPLA6 (14261-1-AP) by Proteintech is a Polyclonal antibody targeting PNPLA6 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 14261-1-AP targets PNPLA6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, SH-SY5Y cells Positive IP detected in: HEK-293 cells Positive IHC detected in: rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information PNPLA6(patatin-like phospholipase domain-containing protein 6) is also named as NTE(neuropathy target esterase) and belongs to the NTE family. It plays an important role in motor neuron development and implicated overexpression of BMP signaling(PMID:22996643). Defects in PNPLA6 are the cause of spastic paraplegia autosomal recessive type 39 (SPG39)(PMID:18313024). This protein has a glycosylaion site that it can be glycosylated(PMID:9576844). It has 3 isoforms produced by alternative splicing. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5546 Product name: Recombinant human PNPLA6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 977-1327 aa of BC051768 Sequence: EERSASRTKQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHKDGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGDSLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPPIDCFKTMDFGKFDQIYDVGYQYGKAVFGGWSRGNVIEKMLTDRRSTDLNESRRADVLAFPSSGFTDLAEIVSRIEPPTSYVSDGCADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEMEEEKSILRQRRCLPQEPPGSATDA Predict reactive species Full Name: patatin-like phospholipase domain containing 6 Calculated Molecular Weight: 150 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC051768 Gene Symbol: PNPLA6 Gene ID (NCBI): 10908 RRID: AB_2283965 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IY17 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.