Proteintech, 14268-1-AP, BMAL1 Polyclonal antibody

Size: 20ul / 150ul The BMAL1 (14268-1-AP) by Proteintech is a Polyclonal antibody targeting BMAL1 in WB, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 14268-1-AP targets BMAL1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-1080 cells, 3T3-L1 cells, HeLa cells, SH-SY5Y cells, mouse liver tissue, MCF-7 cells, NCI-H1299 cells, rat liver tissue Positive IP detected in: HeLa cells Positive IF/ICC detected in: U-87 MG cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5586 Product name: Recombinant human ARNTL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC041129 Sequence: MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESMDTDKDDPHGRLEYTEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFPSTCSKKKDRKSFCTIHSTGYLKSWPPTKMGLDEDNEP Predict reactive species Full Name: aryl hydrocarbon receptor nuclear translocator-like Calculated Molecular Weight: 69 kDa Observed Molecular Weight: 69-75 kDa GenBank Accession Number: BC041129 Gene Symbol: ARNTL Gene ID (NCBI): 406 RRID: AB_2878037 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O00327 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.