Proteintech, 14276-1-AP, CNOT1 Polyclonal antibody

Size: 20ul / 150ul The CNOT1 (14276-1-AP) by Proteintech is a Polyclonal antibody targeting CNOT1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14276-1-AP targets CNOT1 in WB, IHC, IF/ICC, IP, chIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human brain tissue, HEK293 cells, mouse kidney tissue, mouse spleen tissue, mouse thymus tissue, HEK-293 cells, mouse brain tissue, mouse heart tissue, HeLa cells Positive IP detected in: mouse kidney tissue, HeLa cells Positive IHC detected in: human heart tissue, human spleen tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells, sodium arsenite treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms.This is a rabbit polyclonal antibody raised against part chain of C-terminal CNOT1 of human origin. CNOT1 exsits as many isoforms and molecular weight of isoforms are 267, 241 and 173 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5623 Product name: Recombinant human CNOT1 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-354 aa of BC040523 Sequence: MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECALLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSSDLRGFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRRDFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIVQFGVREVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSDGAQAHTWNVEVLIDVLKELNPSLNF Predict reactive species Full Name: CCR4-NOT transcription complex, subunit 1 Calculated Molecular Weight: 267 kDa Observed Molecular Weight: 240-250 kDa, 174 kDa GenBank Accession Number: BC040523 Gene Symbol: CNOT1 Gene ID (NCBI): 23019 RRID: AB_10888627 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: A5YKK6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.