Proteintech, 14316-1-AP, SOD3 Polyclonal antibody

Size: 20ul / 150ul The SOD3 (14316-1-AP) by Proteintech is a Polyclonal antibody targeting SOD3 in WB, IP, ELISA applications with reactivity to human samples 14316-1-AP targets SOD3 in WB, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human urine sample, human heart tissue, human urine, human placenta tissue Positive IP detected in: human placenta tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2. SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5556 Product name: Recombinant human SOD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC014418 Sequence: MLALLCSCLLLAAGASDAWTGEDSAEPNSDSAEWIRDMYAKVTEIWQEVMQRRDDDGTLHAACQVQPSATLDAAQPRVTGVVLFRQLAPRAKLDAFFALEGFPTEPNSSSRAIHVHQFGDLSQGCESTGPHYNPLAVPHPQHPGDFGNFAVRDGSLWRYRAGLAASLAGPHSIVGRAVVVHAGEDDLGRGGNQASVENGNAGRRLACCVVGVCGPGLWERQAREHSERKKRRRESECKAA Predict reactive species Full Name: superoxide dismutase 3, extracellular Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 25-33 kDa GenBank Accession Number: BC014418 Gene Symbol: SOD3 Gene ID (NCBI): 6649 RRID: AB_10898170 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P08294 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.