Proteintech, 14322-1-AP, VWA1 Polyclonal antibody

Size: 20ul / 150ul The VWA1 (14322-1-AP) by Proteintech is a Polyclonal antibody targeting VWA1 in WB, IHC, ELISA applications with reactivity to human samples 14322-1-AP targets VWA1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: T-47D cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information VWA1 (also known as WARP) is a member of the von Willebrand factor A-domain (VA-domain) superfamily of extracellular matrix proteins which may play a role in cartilage structure and function (PMID: 12062410). VWA1 is a multimeric component of the chondrocyte pericellular matrix in articular cartilage and intervertebral disc, where it interacts with the basement membrane heparan sulfate proteoglycan perlecan (PMID: 18314316). VWA1 may have a role in maintaining the blood-brain barrier. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5565 Product name: Recombinant human VWA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-445 aa of BC059409 Sequence: MLPWTALGLALSLRLALARSGAERGPPASAPRGDLMFLLDSSASVSHYEFSRVREFVGQLVAPLPLGTGALRASLVHVGSRPYTEFPFGQHSSGEAAQDAVRASAQRMGDTHTGLALVYAKEQLFAEASGARPGVPKVLVWVTDGGSSDPVGPPMQELKDLGVTVFIVSTGRGNFLELSAAASAPAEKHLHFVDVDDLHIIVQELRGSILDAMRPQQLHATEITSSGFRLAWPPLLTADSGYYVLELVPSAQPGAARRQQLPGNATDWIWAGLDPDTDYDVALVPESNVRLLRPQILRVRTRPGEAGPGASGPESGAGPAPTQLAALPAPEEAGPERIVISHARPRSLRVSWAPALGSAAALGYHVQFGPLRGGEAQRVEVPAGRNCTTLQGLAPGTAYLVTVTAAFRSGRESALSAKACTPDGPRPRPRPVPRAPTPGTASREP Predict reactive species Full Name: von Willebrand factor A domain containing 1 Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 48 kDa GenBank Accession Number: BC059409 Gene Symbol: VWA1 Gene ID (NCBI): 64856 RRID: AB_2241694 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6PCB0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.