Proteintech, 14331-1-AP, PPIP5K1 Polyclonal antibody

Size: 20ul / 150ul The PPIP5K1 (14331-1-AP) by Proteintech is a Polyclonal antibody targeting PPIP5K1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 14331-1-AP targets PPIP5K1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U-251 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PPIP5K1 (Inositol diphosphoinositol pentakisphosphate kinase type 1) plays an important role in regulating cell death in response to genotoxic stress. PPIP5K1 act in concert with IP6K2 to promote cell death through controlling localization of stress-activated MAP kinase-interacting protein1 (SIN1) (PMID: 31051014). The activation of the PtdIns 3-kinase pathway is associated with PPIP5K1 translocation from the cytoplasm to the plasma membrane (PMID: 21222653). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5614 Product name: Recombinant human HISPPD2A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC050263 Sequence: MWSLTASEGESTTAHFFLGAGDEGLGTRGIGMRPEESDSELLEDEEDEVPPEPQIIVGICAMTKKSKSKPMTQILERLCRFDYLTVVILGEDVILNEPVENWPSCHCLISFHSKGFPLDKAVAYSKLRNPFLINDLAMQYYIQDRREVYRILQEEGIDLPRYAVLNRDPARPEECNLIEGEDQVEVNGAVFPKPFVEKPVSAEDHNVYIYYPSSAGGGSQRLFRKIGSRSSVYSPESSVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEIRYPVMLTAME Predict reactive species Full Name: histidine acid phosphatase domain containing 2A Calculated Molecular Weight: 160 kDa Observed Molecular Weight: 160 kDa GenBank Accession Number: BC050263 Gene Symbol: HISPPD2A Gene ID (NCBI): 9677 RRID: AB_3669185 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6PFW1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.