Proteintech, 14338-1-AP, RNF114 Polyclonal antibody

Size: 20ul / 150ul The RNF114 (14338-1-AP) by Proteintech is a Polyclonal antibody targeting RNF114 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 14338-1-AP targets RNF114 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, MCF-7 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: BGC-823 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RNF114, also known as ZNF228 or ZNF313, is a member of the zinc-binding protein family. RNF114 contains two C2H2 and Ring finger zinc-binding domains and a ubiquitin-interacting motif (UIM). RNF114 functions as an E3 ligase in the ubiquitin-proteasome system and plays an important role in basic cellular processes in different types of cells. RNF114 is expressed widely in many normal tissues, such as the heart, liver, and kidney. RNF114 has 2 isoforms with the molecular mass of 21 and 26 kDa. RNF114 is a novel determinant of psoriasis susceptibility, and its overexpression is a crucial indicator of epithelial inflammation (PMID: 34104941, 35069903, 37523804). Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5649 Product name: Recombinant human RNF114 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-228 aa of BC066919 Sequence: MAAQQRDCGGAAQLAGPAAEADPLGRFTCPVCLEVYEKPVQVPCGHVFCSACLQECLKPKKPVCGVCRSALAPGVRAVELERQIESTETSCHGCRKNFFLSKIRSHVATCSKYQNYIMEGVKATIKDASLQPRNVPNRYTFPCPYCPEKNFDQEGLVEHCKLFHSTDTKSVVCPICASMPWGDPNYRSANFREHIQRRHRFSYDTFVDYDVDEEDMMNQVLQRSIIDQ Predict reactive species Full Name: ring finger protein 114 Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 26 kDa GenBank Accession Number: BC066919 Gene Symbol: RNF114 Gene ID (NCBI): 55905 RRID: AB_3085435 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y508 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.