Proteintech, 14418-1-AP, ATP1A1 Polyclonal antibody

Size: 150ul The ATP1A1 (14418-1-AP) by Proteintech is a Polyclonal antibody targeting ATP1A1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications with reactivity to human, mouse, rat samples 14418-1-AP targets ATP1A1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: 37°C incubated HEK-293 cells, 37°C incubated mouse brain tissue, 37°C incubated HeLa cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human cervical cancer tissue, human colon tissue, human liver cancer tissue, human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human ovary tumor tissue, human cervical cancer tissue Positive IF/ICC detected in: Caco-2 cells, HEK-293 cells Positive FC (Intra) detected in: HEK-293 cells Positive CoIP detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)-P: IF-P : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Co-Immunoprecipitation (CoIP): COIP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, chicken, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5763 Product name: Recombinant human ATP1A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 347-681 aa of BC050359 Sequence: TAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDI Predict reactive species Full Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide Calculated Molecular Weight: 113 kDa Observed Molecular Weight: 100-110 kDa GenBank Accession Number: BC050359 Gene Symbol: ATP1A1 Gene ID (NCBI): 476 ENSEMBL Gene ID: ENSG00000163399 RRID: AB_2227873 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P05023 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.